Carboxymethyl Cellulase (CMCase) from UV-irradiation Mutated Bacillus cereus FOA-2 cultivated on Plantain (Musa parasidiaca) Stalk-based Medium: Production, Purification and Characterization
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چکیده
منابع مشابه
Optimization of Culture Conditions and Medium Components for Carboxymethyl Cellulase (CMCase) Production by a Novel Basidiomycete Strain Peniophora sp. NDVN01
Background: Cellulytic enzymes including carboxymethyl cellulases play the key role in hydrolysis of cellulose, a huge organic carbon reservoir on earth, into monomeric sugars and their eventual conversion into valuable chemicals and energy sources. Objectives: In this study, we described the identification of a basidiomycete isolate NDVN01 and optimization of culture conditions and medium com...
متن کاملPurification and characterization of carboxymethyl cellulase from Sinorhizobium fredii
Carboxymethyl cellulase (CMCase) is the key enzyme used by bacteria to decompose the plant root hairwall during symbiosis. In this study, CMCase activities were routinely assayed using carboxymethyl cellulose (CMC) as a substrate. Coupled with the catalytic reaction of BCA (2,2’-bicinchoninic acid) solution, the color developed at 570 nm was measured to determine the enzyme activity. Sinorhizob...
متن کاملoptimization of culture conditions and medium components for carboxymethyl cellulase (cmcase) production by a novel basidiomycete strain peniophora sp. ndvn01
background: cellulytic enzymes including carboxymethyl cellulases play the key role in hydrolysis of cellulose, a huge organic carbon reservoir on earth, into monomeric sugars and their eventual conversion into valuable chemicals and energy sources. objectives: in this study, we described the identification of a basidiomycete isolate ndvn01 and optimization of culture conditions and medium comp...
متن کاملProduction, purification and characterization of a novel thermotolerant endoglucanase (CMCase) from Bacillus strain isolated from cow dung
In an attempt to screen out cellulase producing bacteria from herbivorous animal fecal matter it was possible to isolate a potent bacterium from cow dung. The bacterium was identified as Bacillus sp. using 16S rDNA based molecular phylogenetic approach. The effect of different agricultural wastes, paper wastes and carboxymethyl cellulose on endoglucanase production was tested and was found to p...
متن کاملPRODUCTION AND CHARACTERIZATION OF THERMOPHILIC CARBOXYMETHYL CELLULASE SYNTHESIZED BY Bacillus sp. GROWING ON SUGARCANE BAGASSE IN SUBMERGED FERMENTATION
The production and characterization of cellulase from thermophilic strain Bacillus sp. C1AC5507 was studied. For enzyme production, sugarcane bagasse was used as carbon source. The produced carboxymethyl cellulase (CMCase) had a molecular weight around 55 kDa and its activity varied between 0.14 and 0.37 IU mL in conditions predicted by Response Surface Methodology. The optimum temperature and ...
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ژورنال
عنوان ژورنال: Scientific African
سال: 2021
ISSN: 2468-2276
DOI: 10.1016/j.sciaf.2020.e00691